mouse monoclonal antibodies against ovine il 6  (Bio-Rad)

Journal: eLife

Article Title: Loss of dihydroceramide desaturase drives neurodegeneration by disrupting endoplasmic reticulum and lipid droplet homeostasis in glial cells

doi: 10.7554/eLife.99344

Figure Lengend Snippet: ( A, B ) Abdominal segments of dissected ventral nerve cords from late-third instar larvae of the indicated genotype labeled for fatty acid binding protein (FABP) to label cortex glia and ELAV to label neurons ( A–A’’ ) or FABP and the ER-marker Calnexin-99A (CNX99A; B–B’’ ). As observed in ifc js3 / ifc-KO larvae, ifc js1 and ifc js2 larvae exhibited swollen cortex glia ( A, B ) and an expanded ER phenotype in cortex glia as indicated by elevated CNX99A expression ( B ). ( C–C’’’ ) ifc js1 and ifc js1 / ifc js2 larvae also exhibit enhanced presence of the neuronal cell death marker Cleaved Caspase-3 (CC3). Note that the chromosome that harbors the ifc js2 chromosome also harbors a mutation in the pro-apoptotic gene, Dark . Therefore, to track the impact of ifc js2 on neuronal cell death, we assessed neuronal cell death in larvae trans-heterozygous for ifc js1 and ifc js2 (Civ). Scale bar is 50 µm.

Article Snippet: Antibody , Mouse anti-CNX 99A monoclonal , DSHB , RRID: AB_2722011 , 1:20.

Techniques: Labeling, Binding Assay, Marker, Expressing, Mutagenesis